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〔報文〕竹材から得たフラスを用いて加害種を特定する分子生物学的手法の確立
https://tobunken.repo.nii.ac.jp/records/9152
https://tobunken.repo.nii.ac.jp/records/915264f06a73-42a9-4a66-956f-2ab778f8fee1
名前 / ファイル | ライセンス | アクション |
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06101 (2.4 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2022-04-13 | |||||
タイトル | ||||||
タイトル | 〔報文〕竹材から得たフラスを用いて加害種を特定する分子生物学的手法の確立 | |||||
タイトル | ||||||
タイトル | Molecular Identification of Bamboo-Boring Beetles from Frass by PCR Amplification | |||||
言語 | en | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
篠崎(矢花), 聡子
× 篠崎(矢花), 聡子× 小峰, 幸夫× 島田, 潤× 佐藤, 嘉則× SHINOZAKI-YABANA, Satoko× KOMINE, Yukio× SHIMADA, Megumi× SATO, Yoshinori |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Bamboo has been utilized since ancient times as part of buildings, arts and crafts, tools for classical and folk performing arts, and musical instruments, and there are many Asian cultural properties made of bamboo. However, when bamboo used for a cultural property is damaged by bamboo-boring beetles, it is difficult to maintain it in good condition. An ideal measure would include identification of the bamboo-boring beetles that are actually feeding on bamboo, application of the best control methods based on the ecological information of the pests, and utilization of conservation methods appropriate to each situation to prevent damage by bamboo-boring beetles. The presence of bamboo-boring beetles can be inferred by visually observing the presence of escape holes in the material and the presence of frass in the vicinity. It would be a very effective identification system if the species of bamboo-boring beetles could be identified from their escape holes and frass. In the present study, five major species of insects boring bamboo in Japan ― Lyctus africanus, Lyctoxylon dentatum, Chlorophorus annularis, Dinoderus minutus, and Purpuricenus temminckii ― were selected. The species of the insect samples were identified based on the morphological characteristics. Their COI gene sequences were determined and registered, and specific primers were selected to amplify each COI gene in PCR amplification in order to set up an effective system to specifically identify the target insects. It was found that it is possible to identify the species of bamboo-boring beetles using molecular biological methods by the DNA extraction from frass obtained at cultural property buildings and containing many unknown insects, substances, and contaminants. Finally, the development of the system to identify bamboo-boring beetles from frass made it possible to detect the species without capturing adult insects and is very important for grasping insect damage of cultural properties at an early stage |
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書誌情報 |
保存科学 en : Science for Conservation 号 61, p. 1-12, 発行日 2022-03-17 |
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出版者 | ||||||
出版者 | 独立行政法人国立文化財機構 東京文化財研究所 |