@article{oai:tobunken.repo.nii.ac.jp:00005751,
 author = {佐藤, 嘉則 and 木川, りか and 犬塚, 将英 and 森井, 順之 and 矢島, 國雄 and Yoshinori, SATO and Rika, KIGAWA and Masahide, INUZUKA and Masayuki, MORII and Kunio, YAJIMA},
 issue = {57},
 journal = {保存科学, Science for conservation},
 month = {Mar},
 note = {A processing trace left on the door stone of the Torazuka Tumulus at the time it was made is conserved in the exhibition facility built inside the burial mound of the tumulus. Although a periodic fungicidal treatment had been performed, the surface of the door stone was recently covered with microbial biofilms and fungal mycelium. Microbial colonization may cause not only biodeterioration of valuable stone surface but also disturbance of appreciation for viewers. For these reasons, culture-independent analysis was performed to reveal microbial communities in the biofilm. Furthermore, the respiration rate was measured before and after UV irradiation to assess the effect of the biofilm sterilization.
From the result of culture-independent analysis, it was found that the DNA in the biofilm contained bacterial sequences related to those of Chryseolinea sp. (14%) and Methylacidiphilum sp.(9%)and fungal sequences related to those of Exophiala sp.(95%)and Cladophialophora sp.(7%).It was found that UV irradiation treatment had almost no effect on the biofilm sterilization because the respiration rate was increased after the treatment.
Physical removal by manual labor was eventually chosen for removing the biofilm on the door stone. It was an effective method for decreasing the biofilm directly and for microbiological control in the long term by depleting the source of nutrition for heterotrophic microbes. On the other hand, repeated physical treatment has possibilities for artificial deterioration of the surface of the door stone. It will therefore be necessary to argue studiously about the tool, method, and frequency.},
 pages = {67--76},
 title = {〔報告〕虎塚古墳石室の扉石表面に形成したバイオフィルムの微生物群集構造解析とその制御},
 year = {2018}
}