@article{oai:tobunken.repo.nii.ac.jp:00003926, author = {佐藤, 嘉則 and 西澤, 智康 and 小沼, 奈那美 and 犬塚, 将英 and 森井, 順之 and 木川, りか and 朽津, 信明 and Yoshinori, SATO and Tomoyasu, NISHIZAWA and Nanami, KONUMA and Masahide, INUZUKA and Masayuki, MORII and Rika, KIGAWA and Nobuaki, KUCHITSU}, issue = {56}, journal = {保存科学, Science for conservation}, month = {Mar}, note = {Sekijinsan Tumulus in Hirokawa, Fukuoka prefecture is a keyhole-shaped burial mound with a decorated stone sarcophagus that was built in the first half of the 5th century. Recently, green epiphytic organisms have spread widely on the western side of the sarcophagus surface, preventing preservation and exhibition to the public. The aim of the present study was to analyze the epiphytic microbial community by using molecular techniques to determine the algal, fungal and bacterial community structures on the sarcophagus surface. The algal gene sequences closely related to those of nitrogen-fixing Tolypothrix distorta and salt-tolerant Halospirulina were detected. The environmental factors of limited nitrogen source,solar radiation,and dry and salty conditions were considered to have contributed to the ability of these algae to grow on the sarcophagus surface.Moreover, algal gene sequences closely related to that of Cyanidium were detected. The genus Cyanidium is a red algal extremophile which typically grows in acidic hot springs. It is known that organisms related to such an evolutionarily old red alga are able to grow on the stone sarcophagus surface. In fungal community structure analysis, sequences closely related to Mariannaea elegans and Trichoderma virens were commonly and frequently observed. In bacterial community structure analysis, Arthrobacter russicus, Sphingomonas leidyi and Brevundimonas vesicularis were commonly detected. In the present study,by using a molecular biology approach,the community structures of microorganisms on the stone sarcophagus surface were clarified. However, these community structures are expected to change even under natural conditions,depending on the surrounding environment that is influenced by annual and seasonal fluctuations or the growth of trees. With these changes, it is important to conduct ongoing surveys while continuing studies on biological control methods.}, pages = {1--14}, title = {〔報文〕石人山古墳装飾石棺表面に形成した着生生物群集の構造解析}, year = {2017} }