@article{oai:tobunken.repo.nii.ac.jp:00003905, author = {木川, りか and 雪, 真弘 and 佐藤, 嘉則 and 遠藤, 力也 and 小峰, 幸夫 and 原田, 正彦 and 大熊, 盛也 and Rika, KIGAWA and Masahiro, YUKI and Yoshinori, SATO and Rikiya, ENDOH and Yukio, KOMINE and Masahiko, HARADA and Moriya, OHKUMA}, issue = {54}, journal = {保存科学, Science for conservation}, month = {Mar}, note = {A rare anobiid species in Japan, Priobium cylindricum, was found by chance in the restoration work of the Sambutsu-do of Rinnohji temple in 2008.Severe damage was found in some structural wooden pieces of the temple. The damage was very severe in hard (heart)wood, showing a powdery state and many holes of various sizes. Such severe damage of heart wood is commonly observed in timbers infested by termites, which are known to digest cellulose and hemicellulose effectively with enzymes such as cellulases. From observations of significant damage of heart wood of wooden historic buildings by Priobium cylindricum,we suspected that the anobiid might also utilize enzymes to damage cellulose of wood.Larvae of Priobium cylindricum were collected from infested wooden parts of the Sambutsu-do, and enzymatic activity of crude extract of the insect larvae was measured in comparison with that of a termite species, Hodotermopsis sjostedti, and other common insect species that affect cultural objects,such as Lasioderma serricorne, Attagenus japonicas and Anthrenus verbasci. As a result, crude extracts of the active larvae of Priobium cylindricum showed enzymatic activity of CMCase,xylanase and cellobiosidase.Comparative activity values of CMCase and xylanase were lower than those of a termite of Hodotermopsis sjostedti, but that of cellobiosidase which represents cellulase activity of crystalline cellulose was almost the same as that of Hodotermopsis sjostedti. However,larvae of Priobium cylindricum just before pupation did not show significant activity of such enzymes. This suggests that at least the active larvae of Priobium cylindricum that are infesting wood have detectable enzymatic activity. Larvae and adults of Lasioderma serricorne, larvae of Attagenus japonicas and adults of Anthrenus verbasci did not show significant cellulase activity. Crystalline cellulose content of the powdery parts infested by Priobium cylindricum was measured and compared with the healthy parts of the same timber.The result showed a decrease of crystalline cellulose content in the infested powdery parts, and this seemed to be consistent with the detection of cellulase activities from the active larvae of Priobium cylindricum.}, pages = {145--159}, title = {〔報告〕歴史的木造建造物を加害するオオナガシバンムシ幼虫のセルラーゼ活性について}, year = {2015} }