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〔報告〕分子生物学的手法による高松塚古墳・キトラ古墳の微生物群集構造解析
https://doi.org/10.18953/00005724
https://doi.org/10.18953/000057249a500bfc-9b35-415a-b6a4-26893f6dddb4
名前 / ファイル | ライセンス | アクション |
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05703.pdf (3.0 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2018-05-15 | |||||
タイトル | ||||||
タイトル | 〔報告〕分子生物学的手法による高松塚古墳・キトラ古墳の微生物群集構造解析 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Microbial Community Analyses of the Takamatsuzuka and Kitora Tumuli Using Molecular Methods | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 高松塚古墳 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | キトラ古墳 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 微生物群集構造解析 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 非培養法 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 変性剤濃度勾配電気泳動 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | クローニング | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Takamatsuzuka Tumulus; Kitora Tumulus; microbial community analysis; culture-independent method; DGGE; cloning | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
ID登録 | ||||||
ID登録 | 10.18953/00005724 | |||||
ID登録タイプ | JaLC | |||||
著者 |
西島, 美由紀
× 西島, 美由紀× 安, 光得× 富田, 順子× 喜友名, 朝彦× 佐藤, 嘉則× 木川, りか× 佐野, 千絵× 宇田川, 滋正× 建石, 徹× 杉山, 純多× Miyuki, NISHIJIMA× Kwang-Deuk, An× Junko, TOMITA× Tomohiko, KIYUNA× Yoshinori, SATO× Rika, KIGAWA× Chie, SANO× Shigemasa, UDAGAWA× Toru, TATEISHI× Junta, SUGIYAMA |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Culture-dependent and -independent methods were applied to elucidate the microbiota involved in the biodeterioration of the 1300-year-old mural paintings, plaster walls, and stone chamber interiors of the Takamatsuzuka and Kitora Tumuli. Most isolated microorganisms were also detected as major by PCR-DGGE; however, several bacteria such as the phyla “Acidobacteria”, “Chloroflexi”, and “Cyanobacteria” were detected only via a culture-independent method (PCR-DGGE). Based on the results of this study, it was presumed that the microorganisms, such as bacteria and fungi, and mites and collembolans living in the tumulus environment invaded the stone chamber interiors of both tumuli. Moreover, it was thought that the organic acids (particularly acetic acid) produced from the microorganisms (e.g., Gluconacetobacter bacteria)not only damaged the plaster directly, but also provided nutrients to other microorganisms and enhanced their outbreaks. Major colonizers of the stone chamber interiors were assignable to ubiquitous microorganisms living in the Asuka-mura environment where both tumuli are located. To detect diverse microbiota in the tumulus samples, it was effective to use microbial community analysis using molecular methods combined with conventional isolation and cultivation methods. | |||||
書誌情報 |
保存科学 en : Science for conservation 号 57, p. 23-47, 発行日 2018-03-23 |